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Vaccine. 2011 Mar 9;29(12):2341-8. doi: 10.1016/j.vaccine.2010.11.069. Epub 2011 Jan 25.

Development of a highly immunogenic recombinant candidate vaccine against human chorionic gonadotropin.

Author information

1
Talwar Research Foundation, E-8 Neb Valley, Neb Sarai, New Delhi 110068, India.

Abstract

Human chorionic gonadotropin (hCG) is synthesized soon after fertilization and is essential for embryonic implantation. A vaccine targeting hCG would be an ideal choice for immuno-contraception; an anti-hCG vaccine developed by Talwar et al., has previously undergone Phase II efficacy trials, providing proof of principle. These trials established the threshold levels of bio-neutralizing anti-hCG antibody titers required to prevent pregnancy; however, these titers (>50 ng/ml) were achieved in only 80% of immunized women. In this communication, we report a novel recombinant anti-hCG vaccine which demonstrates improved immunogenicity. hCGβ was genetically fused at C-terminal to the B-subunit of E. coli heat-labile enterotoxin. The recombinant fusion protein (hCGβ-LTB) was expressed in Pichia pastoris and, upon adsorption on Alhydrogel along with Mycobacterium indicus pranii (MIP) as an immuno-modulator, evoked a very high anti-hCG immune response in 100% of immunized BALB/c mice. This recombinant vaccine is expected to reduce cost as well as facilitate production of a molecularly consistent conjugate on a large scale.

PMID:
21272600
DOI:
10.1016/j.vaccine.2010.11.069
[Indexed for MEDLINE]

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