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Tsitologiia. 2010;52(10):863-8.

[Nuclear localization sequence of bovine gamma-interferon provides translocation of recombinant protein to yeast Pichia pastoris cell nucleus].

[Article in Russian]

Abstract

It is known that heterologous protein production in microorganisms has negative influence upon a host cell. In this regard, of particular interest is to know the fate of recombinant proteins in a cell. In this study, it was shown that NLS of bovine gamma-interferon functions in yeast Pichia pastoris cells. The absence of the C-terminal NLS of bovine gamma-interferon leads to cytoplasmic localization of the recombinant protein.

PMID:
21268843
[Indexed for MEDLINE]
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