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J Exp Clin Cancer Res. 2011 Jan 25;30:13. doi: 10.1186/1756-9966-30-13.

The function and mechanism of COX-2 in angiogenesis of gastric cancer cells.

Author information

1
State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, 15 West Changle Road, Xi'an, 710032, PR China.

Abstract

BACKGROUND:

Here we aimed to investigate the effect of COX-2 siRNA on proliferation and angiogenesis of gastric cancer cells.

METHODS:

The gastric cancer cell line SGC7901 was transfected with COX-2 siRNA, then the growth and angiogenesis of cells were detected by in vitro and in vivo assay. Human microarray, RT-PCR and western blot were used to identify differentially expressed angiogenesis-related molecules in cells with decreased expression of COX-2.

RESULTS:

Down-regulation of COX-2 could significantly inhibit the in vitro and in vivo growth of gastric cancer cells, and suppress the migration and tube formation of human umbilical vein endothelial cells. Totally 23 angiogenesis-related molecules were found involved in COX-2-induced angiogenesis suppression. The results of RT-PCR and western blot showed that down-regulation of COX-2 might inhibit VEGF, Flt-1, Flk-1/KDR, angiopoietin-1, tie-2, MMP2 and OPN.

CONCLUSIONS:

COX-2 might mediate tumor angiogenesis and growth, and could be considered as a target for gastric cancer therapy.

PMID:
21266034
PMCID:
PMC3039621
DOI:
10.1186/1756-9966-30-13
[Indexed for MEDLINE]
Free PMC Article

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