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Opt Express. 2011 Jan 3;19(1):353-62. doi: 10.1364/OE.19.000353.

Extended depth-of-field microscopic imaging with a variable focus microscope objective.

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3-D Visualization and Imaging Systems Laboratory, College of Optical Sciences, University of Arizona, Tucson, Arizona 85721, USA.


Increasing the depth-of-field (DOF) while maintaining high resolution imaging has been a classical challenge for conventional microscopes. Extended DOF (EDOF) is especially essential for imaging thick specimens. We present a microscope capable of capturing EDOF images in a single shot. A volumetric optical sampling method is applied by rapidly scanning the focus of a vari-focal microscope objective throughout the extended depths of a thick specimen within the duration of a single detector exposure. An EDOF image is reconstructed by deconvolving the captured image with the response function of the system. Design of a vari-focal objective and algorithms for restoring EDOF images are presented. Proof-of-concept experimental results demonstrate significantly extended DOF compared to the conventional microscope counterparts.


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