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J Microbiol Methods. 2011 Mar;84(3):398-405. doi: 10.1016/j.mimet.2010.12.026. Epub 2011 Jan 22.

An image-based assay for high throughput screening of Giardia lamblia.

Author information

1
Sandler Center for Drug Discovery, University of California, San Francisco, USA. jiri.gut@ucsf.edu

Abstract

Giardia lamblia is a protozoan parasite that causes widespread gastrointestinal illness. Drugs to treat giardiasis are limited, but efforts to discover new anti-giardial compounds are constrained by the lack of a facile system for cell culture and inhibitor testing. We achieved robust and reproducible growth of G. lamblia in 384-well tissue culture plates in a modified TYI-S-33 medium. A high throughput assay for the screening of potential anti-giardial compounds was developed utilizing the WB strain of G. lamblia and automated optical detection of parasites after growth with tested inhibitors. We screened a library of 1600 known bioactive molecules and identified 12 compounds that inhibited growth of G. lamblia at low- or sub-micromolar concentrations. Our high throughput assay should facilitate evaluation of available chemical libraries for novel drugs to treat giardiasis.

PMID:
21262276
DOI:
10.1016/j.mimet.2010.12.026
[Indexed for MEDLINE]

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