Format

Send to

Choose Destination
See comment in PubMed Commons below
Int J Oncol. 2011 Apr;38(4):1129-35. doi: 10.3892/ijo.2011.918. Epub 2011 Jan 21.

Promoter methylation of heat shock protein B2 in human esophageal squamous cell carcinoma.

Author information

1
Department of Otolaryngology, Head and Neck Cancer Research Division, The Johns Hopkins University, School of Medicine, 1550 Orleans Street, CRB II-5M, Baltimore, MD 21231, USA.

Abstract

Hypermethylation of gene promoters and the corresponding loss of gene expression are recognized as a hallmark of human cancer, and DNA methylation has emerged as a promising biomarker for the detection of human esophageal squamous cell carcinoma (ESCC). To identify novel genes methylated in ESCC, we screened 35 candidate genes identified from an oligonucleotide microarray. Among them, the heat shock protein B2 (HSPB2) was methylated in 95.7% (67/70) of primary ESCCs, whereas no methylation was found in normal esophageal tissues from ESCC patients (0%, 0/20). RT-PCR analysis revealed that HSPB2 expression was silenced or weakly expressed in most ESCC cell lines, and re-activated by the demethylating agent 5-aza-2'-deoxy-cytidine. These results indicate that promoter methylation of HSPB2 is one of the causal factors for loss or down-regulation of HSPB2 expression. mRNA expression of HSPB2 in ESCC tissues was significantly down-regulated compared to normal tissues. Our data suggest that promoter methylation of HSPB2 deserves further attention as a novel molecular biomarker in human ESCC.

PMID:
21258768
PMCID:
PMC3309605
DOI:
10.3892/ijo.2011.918
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Spandidos Publications Icon for PubMed Central
    Loading ...
    Support Center