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Biomed Opt Express. 2010 Nov 22;1(5):1472-1479.

Two-parameter angular light scatter collection for microfluidic flow cytometry by unique waveguide structures.

Author information

  • 1Department of Electrical and Computer Engineering, University of California, San Diego, 9500 Gilman Drive MC 0409, La Jolla, CA 92037, USA.

Abstract

Waveguide numerical aperture restrictions and light-blocking elements are used to create a microfluidic cytometer with both illumination and two-parameter light scatter collection systems integrated on-chip. Good forward scatter coefficients of variation (9.7-18.3%) are achieved for polystyrene beads under a reasonably high flow rate (28 cm/s) using a greatly simplified optical system.

PMID:
21258563
PMCID:
PMC3018119
DOI:
10.1364/BOE.1.001472
[PubMed - as supplied by publisher]
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