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Nat Methods. 2011 Mar;8(3):246-9. doi: 10.1038/nmeth.1558. Epub 2011 Jan 23.

Micropilot: automation of fluorescence microscopy-based imaging for systems biology.

Author information

1
Advanced Light Microscopy Facility, European Molecular Biology Laboratory, Heidelberg, Germany.

Abstract

Quantitative microscopy relies on imaging of large cell numbers but is often hampered by time-consuming manual selection of specific cells. The 'Micropilot' software automatically detects cells of interest and launches complex imaging experiments including three-dimensional multicolor time-lapse or fluorescence recovery after photobleaching in live cells. In three independent experimental setups this allowed us to statistically analyze biological processes in detail and is thus a powerful tool for systems biology.

PMID:
21258339
PMCID:
PMC3086017
DOI:
10.1038/nmeth.1558
[Indexed for MEDLINE]
Free PMC Article

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