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Microb Biotechnol. 2009 Nov;2(6):625-33. doi: 10.1111/j.1751-7915.2009.00117.x. Epub 2009 May 21.

Tyramine biosynthesis in Enterococcus durans is transcriptionally regulated by the extracellular pH and tyrosine concentration.

Author information

1
Instituto de Productos Lácteos de Asturias (IPLA), Consejo Superior de Investigaciones Científicas (CSIC), Carretera de Infiesto s/n, 33300 Villaviciosa, Asturias, Spain.

Abstract

The microbial decarboxylation of some amino acids leads to the undesirable presence of biogenic amines in foods. One of the most abundant and frequent biogenic amines found in fermented foods is tyramine, which is produced by the decarboxylation of tyrosine. In the present work, transcriptional analysis of tyramine biosynthesis in Enterococcus durans IPLA655, a strain isolated from cheese, was studied. The gene coding for the tyrosine decarboxylase (tdcA) and that coding for the tyrosine-tyramine antiporter (tyrP) form an operon transcribed from the promoter P(tdcA), the expression of which is regulated by the extracellular pH and tyrosine concentration. Quantification of gene expression during the log phase of growth showed high concentrations of tyrosine and acidic pH conditions to induce tdcA-tyrP polycistronic messenger transcription.

PMID:
21255297
PMCID:
PMC3815318
DOI:
10.1111/j.1751-7915.2009.00117.x
[Indexed for MEDLINE]
Free PMC Article

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