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Eur J Immunol. 1990 Dec;20(12):2661-6.

Cloning and sequencing of human lambda immunoglobulin genes by the polymerase chain reaction.

Author information

1
Molecular Immunopathology Unit, Medical Research Council Centre, Cambridge, GB.

Abstract

Universal oligonucleotide primers, designed for amplifying and sequencing genes encoding the rearranged human lambda immunoglobulin variable region, were validated by amplification of the lambda light chain genes from four human heterohybridoma cell lines and in the generation of a cDNA library of human V lambda sequences from Epstein-Barr virus-transformed human peripheral blood lymphocytes. This technique allows rapid cloning and sequencing of human immunoglobulin genes, and has potential applications in the rescue of unstable human antibody-producing cell lines and in the production of human monoclonal antibodies.

PMID:
2125272
DOI:
10.1002/eji.1830201220
[Indexed for MEDLINE]

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