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J Biol Chem. 2011 Mar 11;286(10):7722-6. doi: 10.1074/jbc.C110.194233. Epub 2011 Jan 18.

Histone deacetylases 9 and 10 are required for homologous recombination.

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1
Department of Biomedical Informatics, Ohio State University Comprehensive Cancer Center, The Ohio State University, Columbus, Ohio 43210, USA.

Abstract

We tested the role of histone deacetylases (HDACs) in the homologous recombination process. A tissue-culture based homology-directed repair assay was used in which repair of a double-stranded break by homologous recombination results in gene conversion of an inactive GFP allele to an active GFP gene. Our rationale was that hyperacetylation caused by HDAC inhibitor treatment would increase chromatin accessibility to repair factors, thereby increasing homologous recombination. Contrary to expectation, treatment of cells with the inhibitors significantly reduced homologous recombination activity. Using RNA interference to deplete each HDAC, we found that depletion of either HDAC9 or HDAC10 specifically inhibited homologous recombination. By assaying for sensitivity of cells to the interstrand cross-linker mitomycin C, we found that treatment of cells with HDAC inhibitors or depletion of HDAC9 or HDAC10 resulted in increased sensitivity to mitomycin C. Our data reveal an unanticipated function of HDAC9 and HDAC10 in the homologous recombination process.

PMID:
21247901
PMCID:
PMC3048658
DOI:
10.1074/jbc.C110.194233
[Indexed for MEDLINE]
Free PMC Article
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