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Proc Natl Acad Sci U S A. 2011 Feb 1;108(5):1903-7. doi: 10.1073/pnas.1019267108. Epub 2011 Jan 18.

Membrane lipidome of an epithelial cell line.

Author information

1
Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany. sampaio@mpi-cbg.de

Abstract

Tissue differentiation is an important process that involves major cellular membrane remodeling. We used Madin-Darby canine kidney cells as a model for epithelium formation and investigated the remodeling of the total cell membrane lipidome during the transition from a nonpolarized morphology to an epithelial morphology and vice versa. To achieve this, we developed a shotgun-based lipidomics workflow that enabled the absolute quantification of mammalian membrane lipidomes with minimal sample processing from low sample amounts. Epithelial morphogenesis was accompanied by a major shift from sphingomyelin to glycosphingolipid, together with an increase in plasmalogen, phosphatidylethanolamine, and cholesterol content, whereas the opposite changes took place during an epithelial-to-mesenchymal transition. Moreover, during polarization, the sphingolipids became longer, more saturated, and more hydroxylated as required to generate an apical membrane domain that serves as a protective barrier for the epithelial sheet.

PMID:
21245337
PMCID:
PMC3033259
DOI:
10.1073/pnas.1019267108
[Indexed for MEDLINE]
Free PMC Article

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