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Curr Opin Cell Biol. 2011 Jun;23(3):310-7. doi: 10.1016/j.ceb.2010.12.004. Epub 2011 Jan 15.

Modern fluorescent proteins and imaging technologies to study gene expression, nuclear localization, and dynamics.

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1
Department of Anatomy and Structural Biology, and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA.

Abstract

Recent developments in reagent design can address problems in single cells that were not previously approachable. We have attempted to foresee what will become possible, and the sorts of biological problems that become tractable with these novel reagents. We have focused on the novel fluorescent proteins that allow convenient multiplexing, and provide for a time-dependent analysis of events in single cells. Methods for fluorescently labeling specific molecules, including endogenously expressed proteins and mRNA have progressed and are now commonly used in a variety of organisms. Finally, sensitive microscopic methods have become more routine practice. This article emphasizes that the time is right to coordinate these approaches for a new initiative on single cell imaging of biological molecules.

PMID:
21242078
PMCID:
PMC3143818
DOI:
10.1016/j.ceb.2010.12.004
[Indexed for MEDLINE]
Free PMC Article

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