[Cloning and sequencing of immunoglobin lambda-chain cDNA in american mink (Mustela vison]

Genetika. 1990 Aug;26(8):1527-31.
[Article in Russian]

Abstract

cDNA library in the lambda gt11 phage was constructed using poly (A+)-mRNA from mink spleen as a template. Immunoscreening of the library allowed the identification of 2 lambda-related clones containing 370 and 803 bp insertion (lambda IGL-1 and lambda IGL-2). Analysis of the primary structure of lambda IGL-2 demonstrated that it contains a large portion of V lambda-segment, J lambda-segment, C lambda-gene and its 3'-untranslated part. The nucleotide sequences known for the immunoglobulin genes were compared to the sequence of the lambda IGL-2 clone. The highest degree of homology was established for the rabbit lambda-genes, this being 63, 94 and 72% for the RF3 region of V lambda-segment, J lambda-segment and C lambda-region, respectively.

Publication types

  • Comparative Study
  • English Abstract

MeSH terms

  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA / genetics*
  • Immunoblotting
  • Immunoglobulin lambda-Chains / genetics*
  • Mink / genetics*
  • Mink / immunology
  • Molecular Sequence Data
  • Rabbits
  • Sequence Homology, Nucleic Acid

Substances

  • Immunoglobulin lambda-Chains
  • DNA