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Nat Methods. 2011 Feb;8(2):135-8. doi: 10.1038/nmeth.1556. Epub 2011 Jan 16.

RNAi screening for fat regulatory genes with SRS microscopy.

Author information

1
Department of Molecular and Human Genetics and Huffington Center on Aging, Baylor College of Medicine, Houston, Texas, USA.

Abstract

Identification of genes regulating fat accumulation is important for basic and medical research; genetic screening for those genes in Caenorhabditis elegans, a widely used model organism, requires in vivo quantification of lipids. We demonstrated RNA interference screening based on quantitative imaging of lipids with label-free stimulated Raman scattering (SRS) microscopy, which overcomes major limitations of coherent anti-Stokes Raman scattering microscopy. Our screening yielded eight new genetic regulators of fat storage.

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PMID:
21240281
PMCID:
PMC3061290
DOI:
10.1038/nmeth.1556
[Indexed for MEDLINE]
Free PMC Article

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