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Lab Chip. 2011 Feb 21;11(4):599-604. doi: 10.1039/c0lc00532k. Epub 2011 Jan 11.

Dielectrophoresis of Caenorhabditis elegans.

Author information

1
Mechanical Engineering and Applied Mechanics, University of Pennsylvania, Philadelphia, PA, USA.

Abstract

We demonstrate for the first time the dielectrophoretic trapping and manipulation of a whole animal, the nematode Caenorhabditis elegans. We studied the effect of the electric field on the nematode as a function of field intensity and frequency. We identified a range of electric field intensities and frequencies that trap worms without apparent adverse effect on their viability. Worms tethered by dielectrophoresis (DEP) exhibit behavioral responses to blue light, indicating that at least some of the nervous system functions are unimpaired by the electrical field. DEP is useful to dynamically tether nematodes, sort nematodes according to size, and separate dead worms from live ones.

PMID:
21225036
PMCID:
PMC3247157
DOI:
10.1039/c0lc00532k
[Indexed for MEDLINE]
Free PMC Article

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