Format

Send to

Choose Destination
Bioconjug Chem. 2011 Feb 16;22(2):156-68. doi: 10.1021/bc100149g. Epub 2011 Jan 11.

Modular construction of multifunctional bioresponsive cell-targeted nanoparticles for gene delivery.

Author information

1
School of Pharmacy and School of Chemistry, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom.

Abstract

Multifunctional and modular block copolymers prepared from biocompatible monomers and linked by a bioreducible disulfide linkage have been prepared using a combination of ring-opening and atom-transfer radical polymerizations (ATRP). The presence of terminal functionality via ATRP allowed cell-targeting folic acid groups to be attached in a controllable manner, while the block copolymer architecture enabled well-defined nanoparticles to be prepared by a water-oil-water double emulsion procedure to encapsulate DNA with high efficiency. Gene delivery assays in a Calu-3 cell line indicated specific folate-receptor-mediated uptake of the nanoparticles, and triggered release of the DNA payload via cleavage of the disulfide link resulted in enhanced transgene expression compared to nonbioreducible analogues. These materials offer a promising and generic means to deliver a wide variety of therapeutic payloads to cells in a selective and tunable way.

PMID:
21222431
DOI:
10.1021/bc100149g
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for American Chemical Society
Loading ...
Support Center