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Mol Microbiol. 1990 Jul;4(7):1215-8.

Generation of capsule-deficient Neisseria meningitidis strains by homologous recombination.

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Medizinische Hochschule Hannover, Institut für Medizinische Mikrobiologie, FRG.


Capsule-deficient mutants of Neisseria meningitidis serogroup B strain B1940 were constructed by allelic replacement using the plasmids pMF120 and pMF121, which contain the flanking regions of the gene locus for the biosynthesis pathway of the group B meningococcal capsular polysaccharide. Southern blot analysis of chromosomal DNA of the capsule-deficient meningococcal strains confirmed the generation of large deletions in the chromosomal cps gene complex. The same strategy proved useful in constructing meningococcal strains with capsular types A, C, W135, Y and Z.

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