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Arch Ophthalmol. 2011 May;129(5):555-61. doi: 10.1001/archophthalmol.2010.344. Epub 2011 Jan 10.

Anterior keratocyte depletion in fuchs endothelial dystrophy.

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1
Department of Ophthalmology, Mayo Clinic, 200 First St SW, Rochester, MN 55905, USA.

Abstract

OBJECTIVE:

To determine if keratocyte populations are different in corneas with Fuchs dystrophy compared with control corneas.

METHODS:

Eleven corneas excised during penetrating keratoplasty for Fuchs dystrophy and 5 control corneas of eyes enucleated for choroidal melanoma were examined using light microscopy. Twenty control corneas age-matched to the corneas with Fuchs dystrophy were examined using confocal microscopy in vivo. The number of keratocytes in a full-thickness column of central stroma with frontal area of 1 mm(2), determined using histologic and confocal methods, was compared between corneas with Fuchs dystrophy and controls.

RESULTS:

By histology, the mean (SD) number of cells in a full-thickness column of stroma in Fuchs dystrophy (12 215 [1394] cells) was less than in control corneas (15 628 [710] cells; P < .001). The mean (SD) number of keratocytes in the anterior 10% of the stroma of corneas with Fuchs dystrophy (682 [274] cells) was less than in the control corneas measured using histology (1858 [404] cells; P < .001) and confocal microscopy (1481 [397] cells; P < .001).

CONCLUSIONS:

Keratocytes are depleted by 54% to 63% in the anterior 10% of the stroma of corneas that require penetrating keratoplasty for Fuchs dystrophy. Keratocyte loss might contribute to anterior stromal changes that persist and degrade vision after endothelial keratoplasty.

PMID:
21220622
PMCID:
PMC3902089
DOI:
10.1001/archophthalmol.2010.344
[Indexed for MEDLINE]
Free PMC Article
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