Format

Send to

Choose Destination
World J Gastroenterol. 2011 Jan 7;17(1):79-88. doi: 10.3748/wjg.v17.i1.79.

MiRNA profile in esophageal squamous cell carcinoma: downregulation of miR-143 and miR-145.

Author information

1
Department of Biochemistry and Molecular Biology, Medical College of Shantou University, Shantou 515041, Guangdong Province, China.

Abstract

AIM:

To investigate the expression profile of miRNA in esophageal squamous cell carcinoma (ESCC).

METHODS:

The expression profile of miRNA in ESCC tissues was analyzed by miRNA microarray. The expression levels of miR-143 and miR-145 in 86 ESCC patients were determined by real-time polymerase chain reaction (PCR) using TaqMan assay. The mobility effect was estimated by wound-healing using esophageal carcinoma cells transfected with miRNA expression plasmids.

RESULTS:

A set of miRNAs was found to be deregulated in the ESCC tissues, and the expression levels of miR-143 and -145 were significantly decreased in most of the ESCC tissues examined. Both miR-143 and miR-145 expression correlated with tumor invasion depth. The transfection of human esophageal carcinoma cells with miR-143 and miR-145 expression plasmids resulted in a greater inhibition of cell mobility, however, the protein level of the previously reported target of miR-145, FSCN1, did not show any significant downregulation.

CONCLUSION:

These findings suggest that the deregulation of miRNAs plays an important role in the progression of ESCC. Both miR-143 and miR-145 might act as anti-oncomirs common to ESCC.

KEYWORDS:

Esophageal squamous cell carcinoma; MicroRNA; Tumor invasion depth; miR-143; miR-145

PMID:
21218087
PMCID:
PMC3016684
DOI:
10.3748/wjg.v17.i1.79
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Baishideng Publishing Group Inc. Icon for PubMed Central
Loading ...
Support Center