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Nat Methods. 2011 Feb;8(2):139-42. doi: 10.1038/nmeth.1552. Epub 2011 Jan 9.

Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing.

Author information

1
Department of Physics and Astronomy, University of California Los Angeles, Los Angeles, California, USA.

Abstract

In vivo two-photon calcium imaging would benefit from the use of multiple excitation beams to increase scanning speed, signal-to-noise ratio and field of view or to image different axial planes simultaneously. Using spatiotemporal multiplexing we circumvented light-scattering ambiguity inherent to deep-tissue multifocal two-photon microscopy. We demonstrate calcium imaging at multiple axial planes in the intact mouse brain to monitor network activity of ensembles of cortical neurons in three spatial dimensions.

PMID:
21217749
PMCID:
PMC3076599
DOI:
10.1038/nmeth.1552
[Indexed for MEDLINE]
Free PMC Article

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