Format

Send to

Choose Destination
See comment in PubMed Commons below
Vaccine. 2011 Feb 11;29(8):1624-7. doi: 10.1016/j.vaccine.2010.12.082. Epub 2011 Jan 5.

Vaccine candidate P6 of nontypable Haemophilus influenzae is not a transmembrane protein based on protein structural analysis.

Author information

1
Department of Chemistry, Rochester Institute of Technology, Rochester, NY, USA.

Abstract

P6 has been a vaccine candidate for nontypable Haemophilus influenzae (NTHi) based on its location on the outer membrane and immunogenicity. Because P6 is attached to the inner peptidoglycan layer of NTHi, and is putatively surface exposed, it must be a transmembrane protein. We examined the P6 structure using computational modeling, site-directed mutagenesis, and nuclear magnetic resonance spectroscopy. We found that P6 cannot be a transmembrane protein, and therefore may not be surface exposed. We conclude that there may be another protein on the surface of NTHi that has epitopes similar if not identical to P6.

PMID:
21215345
PMCID:
PMC3070289
DOI:
10.1016/j.vaccine.2010.12.082
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Support Center