Format

Send to

Choose Destination
Biomol NMR Assign. 2011 Oct;5(2):151-5. doi: 10.1007/s12104-010-9289-7. Epub 2011 Jan 7.

Sequence-specific 1H, 13C and 15N assignments of the phosphoesterase (PE) domain of Pseudomonas aeruginosa DNA ligase D (LigD).

Author information

1
The New York Structural Biology Center, 89 Convent Avenue, New York, NY 10027, USA.

Abstract

DNA ligase D (LigD), consisting of polymerase, ligase and phosphoesterase domains, is the essential catalyst of the bacterial non-homologous end-joining pathway of DNA double-strand break repair. The phosphoesterase (PE) module performs manganese-dependent 3'-phosphomonoesterase and 3'-ribonucleoside resection reactions that heal broken ends in preparation for sealing. LigD PE exemplifies a structurally and mechanistically unique class of DNA end-processing enzymes. Here, we present the resonance assignments of the PE domain of Pseudomonas aeruginosa LigD comprising the N-terminal 177 residues.

PMID:
21213076
PMCID:
PMC4156853
DOI:
10.1007/s12104-010-9289-7
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Springer Icon for PubMed Central
Loading ...
Support Center