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Cell Biochem Biophys. 2011 Apr;59(3):179-84. doi: 10.1007/s12013-010-9130-z.

Induction of human bone marrow mesenchymal stem cells differentiation into neural-like cells using cerebrospinal fluid.

Author information

1
Department of Anesthesiology, the first Affiliated Hospital, China Medical University, Shenyang, 110001, China. xzmcyy@163.com

Abstract

To optimize a technique that induces bone marrow mesenchymal stem cells (BMSCs) to differentiation into neural-like cells, using cerebrospinal fluid (CSF) from the patient. In vitro, CSF (Group A) and the cell growth factors EGF and bFGF (Group B) were used to induce BMSCs to differentiate into neural-like cells. Post-induction, presence of neural-like cells was confirmed through the use of light and immunofluorescence microscopy. BMSCs can be induced to differentiate into neural-like cells. The presence of neural-like cells was confirmed via morphological characteristics, phenotype, and biological properties. Induction using CSF can shorten the production time of neural-like cells and the quantity is significantly higher than that obtained by induction with growth factor (P < 0.01). The two induction methods can induce BMSCs to differentiate into neural-like cells. Using CSF induction, 30 ml bone marrow can produce a sufficient number of neural-like cells that totally meet the requirements for clinical treatment.

PMID:
21210249
DOI:
10.1007/s12013-010-9130-z
[Indexed for MEDLINE]

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