Immunoblots of parkin immunoprecipitates (2 mg) prepared from lysates of (a) TX-100-soluble striatum, (c) TX-100-insoluble striatum, and (e) TX-100-soluble cortex lysates. Brain tissue lysates (100 μg) were immunoblotted with antibodies to parkin, AIMP2, FBP-1, ubiquitin (Ub), P-tyr, P-c-Abl (p-Y245), c-Abl, and actin. Ratios of phospho-parkin/IP parkin and AIMP2/actin from lysates of (b) TX-100-soluble striatum, (d) TX-100-insoluble striatum, and (f) TX-100-soluble cortex for control versus PD (* p < 0.05, Student's t-test). OxyBlot analysis of proteins from post-mortem (g) striatum, (h) cortex of PD patients and age-matched controls. All experiments were repeated at least three times. Representative examples are presented.