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Genome Biol. 2011;12(1):R1. doi: 10.1186/gb-2011-12-1-r1. Epub 2011 Jan 4.

A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries.

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1
Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, MA 02141, USA.

Abstract

Genome targeting methods enable cost-effective capture of specific subsets of the genome for sequencing. We present here an automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready libraries produced. Significant process improvements and a series of in-process quality control checkpoints are also added. These process improvements can also be used in a manual version of the protocol.

PMID:
21205303
PMCID:
PMC3091298
DOI:
10.1186/gb-2011-12-1-r1
[Indexed for MEDLINE]
Free PMC Article
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