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Biochem J. 2011 Mar 1;434(2):211-7. doi: 10.1042/BJ20101772.

Male infertility-linked point mutation disrupts the Ca2+ oscillation-inducing and PIP(2) hydrolysis activity of sperm PLCζ.

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1
Cell Signalling Laboratory, Wales Heart Research Institute, Cardiff University School of Medicine, Cardiff CF14 4XN, UK.

Erratum in

  • Biochem J. 2011 May 1;435(3):791. Nounesis, Georg [corrected to Nounesis, George].

Abstract

A male infertility-linked human PLCζ (phospholipase Cζ) mutation introduced into mouse PLCζ completely abolishes both in vitro PIP(2) (phosphatidylinositol 4,5-bisphosphate) hydrolysis activity and the ability to trigger in vivo Ca2+ oscillations in mouse eggs. Wild-type PLCζ initiated a normal pattern of Ca2+ oscillations in eggs in the presence of 10-fold higher mutant PLCζ, suggesting that infertility is not mediated by a dominant-negative mechanism.

PMID:
21204786
PMCID:
PMC3195387
DOI:
10.1042/BJ20101772
[Indexed for MEDLINE]
Free PMC Article
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