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Chimia (Aarau). 2010;64(11):789-92.

IMAPlate based miniature, high sensitive, rapid screening method for detecting bioengineered, secreted lipase activities in yeast expression systems.

Author information

1
Hochschule für Life Sciences, Fachhochschule Nordwestschweiz Grandenstrasse 40, Muttenz.

Abstract

A spectrophotometric assay based on a miniaturized 96-well plate device (IMAPlate) enables a rapid and simple screening of bioengineered recombinant lipases expressed and secreted by Saccharomyces cerevisiae. Starting from a colony, the test delivers a quantitative estimation of enzymatic activity titer in 24 h or less with manual high throughput performances.

PMID:
21197842
DOI:
10.2533/chimia.2010.789
[Indexed for MEDLINE]

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