Format

Send to

Choose Destination
J Biol Chem. 2011 Mar 4;286(9):7601-8. doi: 10.1074/jbc.M110.190108. Epub 2011 Jan 1.

Quantitative mapping of reversible mitochondrial Complex I cysteine oxidation in a Parkinson disease mouse model.

Author information

1
Buck Institute for Age Research, Novato, California 94945, USA.

Abstract

Differential cysteine oxidation within mitochondrial Complex I has been quantified in an in vivo oxidative stress model of Parkinson disease. We developed a strategy that incorporates rapid and efficient immunoaffinity purification of Complex I followed by differential alkylation and quantitative detection using sensitive mass spectrometry techniques. This method allowed us to quantify the reversible cysteine oxidation status of 34 distinct cysteine residues out of a total 130 present in murine Complex I. Six Complex I cysteine residues were found to display an increase in oxidation relative to controls in brains from mice undergoing in vivo glutathione depletion. Three of these residues were found to reside within iron-sulfur clusters of Complex I, suggesting that their redox state may affect electron transport function.

PMID:
21196577
PMCID:
PMC3045014
DOI:
10.1074/jbc.M110.190108
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center