Format

Send to

Choose Destination
Methods Mol Biol. 2011;709:141-51. doi: 10.1007/978-1-61737-982-6_9.

Systemic gene transfer to skeletal muscle using reengineered AAV vectors.

Author information

1
Gene Therapy Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Abstract

Gene therapy of musculoskeletal disorders warrants efficient gene transfer to a wide range of muscle groups. Reengineered adeno-associated viral (AAV) vectors that selectively transduce muscle tissue following systemic administration are attractive candidates for such applications. Here we provide examples of several lab-derived AAV vectors that display systemic tissue tropism in mice. Methods to evaluate the efficiency of gene transfer to skeletal muscle following intravenous or isolated limb infusion of AAV -vectors in mice are discussed in detail.

PMID:
21194026
PMCID:
PMC3253371
DOI:
10.1007/978-1-61737-982-6_9
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Springer Icon for PubMed Central
Loading ...
Support Center