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Nat Rev Genet. 2011 Feb;12(2):87-98. doi: 10.1038/nrg2934. Epub 2010 Dec 30.

RNA sequencing: advances, challenges and opportunities.

Author information

1
Helicos BioSciences Corporation, One Kendall Square, Cambridge, Massachusetts 02139, USA. fozsolak@helicosbio.com

Abstract

In the few years since its initial application, massively parallel cDNA sequencing, or RNA-seq, has allowed many advances in the characterization and quantification of transcriptomes. Recently, several developments in RNA-seq methods have provided an even more complete characterization of RNA transcripts. These developments include improvements in transcription start site mapping, strand-specific measurements, gene fusion detection, small RNA characterization and detection of alternative splicing events. Ongoing developments promise further advances in the application of RNA-seq, particularly direct RNA sequencing and approaches that allow RNA quantification from very small amounts of cellular materials.

PMID:
21191423
PMCID:
PMC3031867
DOI:
10.1038/nrg2934
[Indexed for MEDLINE]
Free PMC Article

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