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J Clin Microbiol. 2011 Mar;49(3):1137-9. doi: 10.1128/JCM.01831-10. Epub 2010 Dec 29.

Rapid detection of Mycobacterium tuberculosis complex and rifampin resistance in smear-negative clinical samples by use of an integrated real-time PCR method.

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1
Department of Microbiology, IDIBELL-Bellvitge Hospital, C/ Feixa Llarga s/n. 08907 Hospitalet de Llobregat, Barcelona, Spain.

Abstract

Sixty-four of 85 (75.3%) smear-negative respiratory (n = 78) and nonrespiratory (n = 7) samples with positive cultures of Mycobacterium tuberculosis complex (MTC) were detected by the GeneXpert system using the Xpert MTB/RIF assay (GX). In addition, GX found rpoB mutations in all six of the rifampin-resistant strains detected. The test was negative in 20 culture-negative and 20 nontuberculous culture-positive samples (100% specificity). GX offers high potential for the diagnosis of tuberculosis due to its capacity for direct detection of MTC, its rapidity, and its simplicity.

PMID:
21191053
PMCID:
PMC3067676
DOI:
10.1128/JCM.01831-10
[Indexed for MEDLINE]
Free PMC Article
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