Format

Send to

Choose Destination
J Proteome Res. 2011 Mar 4;10(3):1416-9. doi: 10.1021/pr1011476. Epub 2011 Feb 3.

Coomassie staining as loading control in Western blot analysis.

Author information

1
Department of Oncology, Clinical Sciences, Lund University, Sweden.

Abstract

In Western blotting, immunodetection of housekeeping proteins is routinely performed to detect differences in electrophoresis loading. The present work describes a much faster and simpler protein staining method, which is compatible with ordinary blocking conditions. In addition, the method can be used after immunodetection with superior linearity compared to ordinary staining methods. After immunoblotting and staining, protein bands can be further identified using peptide mass fingerprinting.

PMID:
21186791
DOI:
10.1021/pr1011476
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for American Chemical Society
Loading ...
Support Center