Format

Send to

Choose Destination
EMBO J. 2011 Mar 2;30(5):859-72. doi: 10.1038/emboj.2010.332. Epub 2010 Dec 24.

Gcn4p-mediated transcriptional repression of ribosomal protein genes under amino-acid starvation.

Author information

1
Laboratory of Biochemistry, School of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.

Abstract

Gcn4p is a well-characterized bZIP transcription factor that activates more than 500 genes encoding amino acids and purine biosynthesis enzymes, and many stress-response genes under various stress conditions. Under these stresses, it had been shown that transcriptions of ribosomal protein (RP) genes were decreased. However, the detailed mechanism of this downregulation has not been elucidated. In this study, we present a novel mechanistic model for a repressive role of Gcn4p on RP transcription, especially under amino-acid starvation. It was found that Gcn4p bound directly to Rap1p, which in turn inhibited Esa1p-Rap1p binding. The inhibition of Esa1p recruitment to RP promoters ultimately reduced the level of histone H4 acetylation and RP transcription. These data revealed that Gcn4p has simultaneous dual roles as a repressor for RP genes as well as an activator for amino-acid biosynthesis genes. Moreover, our results showed evidence of a novel link between general control of amino-acid biosynthesis and ribosome biogenesis mediated by Gcn4p at an early stage of adaptation to amino-acid starvation.

PMID:
21183953
PMCID:
PMC3049204
DOI:
10.1038/emboj.2010.332
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Wiley Icon for PubMed Central
Loading ...
Support Center