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Bioinformatics. 2011 Mar 1;27(5):715-7. doi: 10.1093/bioinformatics/btq707. Epub 2010 Dec 23.

CompleteMOTIFs: DNA motif discovery platform for transcription factor binding experiments.

Author information

1
Children's Hospital Informatics Program at the Harvard-MIT, Division of Health Sciences and Technology, Department of Medicine, Children's Hospital Boston, Boston, MA 02115, USA.

Abstract

CompleteMOTIFs (cMOTIFs) is an integrated web tool developed to facilitate systematic discovery of overrepresented transcription factor binding motifs from high-throughput chromatin immunoprecipitation experiments. Comprehensive annotations and Boolean logic operations on multiple peak locations enable users to focus on genomic regions of interest for de novo motif discovery using tools such as MEME, Weeder and ChIPMunk. The pipeline incorporates a scanning tool for known motifs from TRANSFAC and JASPAR databases, and performs an enrichment test using local or precalculated background models that significantly improve the motif scanning result. Furthermore, using the cMOTIFs pipeline, we demonstrated that multiple transcription factors could cooperatively bind to the upstream of important stem cell differentiation regulators.

AVAILABILITY:

http://cmotifs.tchlab.org.

PMID:
21183585
PMCID:
PMC3105477
DOI:
10.1093/bioinformatics/btq707
[Indexed for MEDLINE]
Free PMC Article

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