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J Microbiol Methods. 2011 Feb;84(2):355-8. doi: 10.1016/j.mimet.2010.12.013. Epub 2010 Dec 21.

Development of a URA5 integrative cassette for gene disruption in the Candida guilliermondii ATCC 6260 strain.

Author information

1
Biomolécules et Biotechnologies Végétales, Faculté de Pharmacie, Université François Rabelais, Tours, France.

Abstract

We designed an efficient transformation system for Candida guilliermondii based on a ura5 ATCC 6260 derived recipient strain and a URA5 recyclable selection marker. This "URA5 blaster" disruption system represents a powerful tool to study the function of a large pallet of genes in this yeast of clinical and biotechnological interest.

PMID:
21182877
DOI:
10.1016/j.mimet.2010.12.013
[Indexed for MEDLINE]

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