Format

Send to

Choose Destination
Anal Chem. 2011 Feb 1;83(3):933-9. doi: 10.1021/ac102658h. Epub 2010 Dec 23.

High throughput assay of diffusion through Cx43 gap junction channels with a microfluidic chip.

Author information

1
Department of Mechanical and Aerospace Engineering, SUNY-Buffalo, Buffalo, New York 14260, USA.

Abstract

This paper describes a microfluidic-based assay capable of measuring gap-junction mediated dye diffusion in cultured cells. The technique exploits multistream laminar flow to selectively expose cells to different environments, enabling continuous loading of cells in one compartment while monitoring, in real time, dye diffusion into cells of a neighboring compartment. A simple one-dimensional diffusion model fit to the data extracted the diffusion coefficient of four different dyes, 5-(6)-carboxyfluorescein, 5-chloromethylfluorescein, Oregon green 488 carboxylic acid, and calcein. Different inhibitors were assayed for their ability to reduce dye coupling. The chip can screen multiple inhibitors in parallel in the same cell preparation, demonstrating its potential for high throughput. The technique provides a convenient method to measure gap junction mediated diffusion and a screen for drugs that affect gap junction communication.

PMID:
21182279
PMCID:
PMC3059360
DOI:
10.1021/ac102658h
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for American Chemical Society Icon for PubMed Central
Loading ...
Support Center