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Methods Mol Biol. 2011;701:67-96. doi: 10.1007/978-1-61737-957-4_4.

Chromosome painting for plant biotechnology.

Author information

1
Division of Biological Sciences, University of Missouri, Columbia, MO, USA.

Abstract

Fluorescence in situ hybridization (FISH) is an invaluable tool for chromosome analysis and engineering. The ability to visually localize endogenous genes, transposable elements, transgenes, naturally occurring organellar DNA insertions - essentially any unique sequence larger than 2 kb - greatly facilitates progress. This chapter details the labeling procedures and chromosome preparation techniques used to produce high-quality FISH signals on somatic metaphase and meiotic pachytene spreads.

PMID:
21181525
DOI:
10.1007/978-1-61737-957-4_4
[Indexed for MEDLINE]

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