Format

Send to

Choose Destination
Virulence. 2010 Nov-Dec;1(6):475-82. Epub 2010 Nov 1.

Methods for using Galleria mellonella as a model host to study fungal pathogenesis.

Author information

1
Division of Infectious, Massachusetts General Hospital, Boston, MA, USA. hfuchs@partners.org

Abstract

The facile inoculum delivery and handling of the insect Galleria mellonella make it a desirable model for the study of fungal pathogenesis. Here we present methods to study fungal virulence, filamentation and fungal cell associates with insect hemocytes using Candida albicans and Cryptococcus neoformans to illustrate the use of this model. The two types of fungi cause distinct infections thus we compare and contrast the infection characteristics observed in G. mellonella. The protocols presented herein can be adapted to the study of other fungal pathogens using G. mellonella as an infection model.

PMID:
21178491
DOI:
10.4161/viru.1.6.12985
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Taylor & Francis
Loading ...
Support Center