Format

Send to

Choose Destination
Nanotechnology. 2011 Feb 4;22(5):055709. doi: 10.1088/0957-4484/22/5/055709. Epub 2010 Dec 23.

Temperature-dependent phase transitions in zeptoliter volumes of a complex biological membrane.

Author information

1
The Center for Nanophase Materials Sciences, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA.

Abstract

Phase transitions in purple membrane have been a topic of debate for the past two decades. In this work we present studies of a reversible transition of purple membrane in the 50-60 °C range in zeptoliter volumes under different heating regimes (global heating and local heating). The temperature of the reversible phase transition is 52 ± 5 °C for both local and global heating, supporting the hypothesis that this transition is mainly due to a structural rearrangement of bR molecules and trimers. To achieve high resolution measurements of temperature-dependent phase transitions, a new scanning probe microscopy-based method was developed. We believe that our new technique can be extended to other biological systems and can contribute to the understanding of inhomogeneous phase transitions in complex systems.

PMID:
21178256
DOI:
10.1088/0957-4484/22/5/055709
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for IOP Publishing Ltd.
Loading ...
Support Center