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J Cell Biol. 2010 Dec 27;191(7):1251-60. doi: 10.1083/jcb.201005065. Epub 2010 Dec 20.

HURP permits MTOC sorting for robust meiotic spindle bipolarity, similar to extra centrosome clustering in cancer cells.

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  • 1Unité Mixte de Recherche 7622, Centre National de la Recherche Scientifique/Université Pierre et Marie Curie, 75005 Paris, France.

Abstract

In contrast to somatic cells, formation of acentriolar meiotic spindles relies on the organization of microtubules (MTs) and MT-organizing centers (MTOCs) into a stable bipolar structure. The underlying mechanisms are still unknown. We show that this process is impaired in hepatoma up-regulated protein (Hurp) knockout mice, which are viable but female sterile, showing defective oocyte divisions. HURP accumulates on interpolar MTs in the vicinity of chromosomes via Kinesin-5 activity. By promoting MT stability in the spindle central domain, HURP allows efficient MTOC sorting into distinct poles, providing bipolarity establishment and maintenance. Our results support a new model for meiotic spindle assembly in which HURP ensures assembly of a central MT array, which serves as a scaffold for the genesis of a robust bipolar structure supporting efficient chromosome congression. Furthermore, HURP is also required for the clustering of extra centrosomes before division, arguing for a shared molecular requirement of MTOC sorting in mammalian meiosis and cancer cell division.

PMID:
21173113
PMCID:
PMC3010075
DOI:
10.1083/jcb.201005065
[PubMed - indexed for MEDLINE]
Free PMC Article
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