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J Cell Biol. 2010 Dec 27;191(7):1261-9. doi: 10.1083/jcb.201003091. Epub 2010 Dec 20.

Cdc42 localization and cell polarity depend on membrane traffic.

Author information

1
Cell Polarity and Migration Group, Institut Pasteur, and Centre National de la Recherche Scientifique URA 2582, 75724 Paris, Cedex 15, France.

Abstract

Cell polarity is essential for cell division, cell differentiation, and most differentiated cell functions including cell migration. The small G protein Cdc42 controls cell polarity in a wide variety of cellular contexts. Although restricted localization of active Cdc42 seems to be important for its distinct functions, mechanisms responsible for the concentration of active Cdc42 at precise cortical sites are not fully understood. In this study, we show that during directed cell migration, Cdc42 accumulation at the cell leading edge relies on membrane traffic. Cdc42 and its exchange factor βPIX localize to intracytosplasmic vesicles. Inhibition of Arf6-dependent membrane trafficking alters the dynamics of Cdc42-positive vesicles and abolishes the polarized recruitment of Cdc42 and βPIX to the leading edge. Furthermore, we show that Arf6-dependent membrane dynamics is also required for polarized recruitment of Rac and the Par6-aPKC polarity complex and for cell polarization. Our results demonstrate influence of membrane dynamics on the localization and activation of Cdc42 and consequently on directed cell migration.

PMID:
21173111
PMCID:
PMC3010071
DOI:
10.1083/jcb.201003091
[Indexed for MEDLINE]
Free PMC Article

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