Format

Send to

Choose Destination
EMBO J. 2011 Jan 19;30(2):263-76. doi: 10.1038/emboj.2010.314. Epub 2010 Dec 17.

Chromatin condensation via the condensin II complex is required for peripheral T-cell quiescence.

Author information

1
Department of Biochemistry, St Jude Children's Research Hospital, Memphis, TN, USA.

Abstract

Naive T cells encountering their cognate antigen become activated and acquire the ability to proliferate in response to cytokines. Stat5 is an essential component in this response. We demonstrate that Stat5 cannot access DNA in naive T cells and acquires this ability only after T-cell receptor (TCR) engagement. The transition is not associated with changes in DNA methylation or global histone modification but rather chromatin decondensation. Condensation occurs during thymocyte development and proper condensation is dependent on kleisin-β of the condensin II complex. Our findings suggest that this unique chromatin condensation, which can affect interpretations of chromatin accessibility assays, is required for proper T-cell development and maintenance of the quiescent state. This mechanism ensures that cytokine driven proliferation can only occur in the context of TCR stimulation.

PMID:
21169989
PMCID:
PMC3025460
DOI:
10.1038/emboj.2010.314
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Wiley Icon for PubMed Central
Loading ...
Support Center