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In Vivo. 2010 Nov-Dec;24(6):861-4.

Urine and serum analysis of consumed curcuminoids using an IkappaB-luciferase surrogate marker assay.

Author information

1
800 Research Parkway Suite 350, Oklahoma City, OK, 73104, U.S.A. ramar@swaasth.com.

Abstract

BACKGROUND:

curcumin metabolites are detectable in body fluids such as serum and urine. We have developed a novel assay that can detect metabolites in such body fluids by measuring their effect on the nuclear factor kappa B/inhibitor of kappa B (NF-κB/IκB) pathway.

PATIENTS AND METHODS:

fifteen healthy individuals were enrolled in the study and randomly assigned to two groups: control group (five) and curcumin group (ten). The test group ingested 8 g of the curcuminoids (C(3)-Complex) with 16 oz of bottled water. Blood and urine were collected at 0, 4, 8, and 24 h after ingestion. Degradation of the NF-κB/IκB complex was detected by the Genetic Expression and Measurement (GEM) assay using HCT116 cells stably transfected with PGL3-IκB firefly luciferase.

RESULTS:

using our novel GEM assay, the five controls who had not taken curcumin were identified.

CONCLUSION:

the GEM assay is a very sensitive and accurate non-invasive assay that could be utilized to detect metabolites in body fluids. It could also serve as a tool to determine participants' compliance during clinical research studies.

PMID:
21164045
PMCID:
PMC3050505
[Indexed for MEDLINE]
Free PMC Article
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