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Gene. 1990 May 31;90(1):43-9.

Construction of lacZ promoter probe vectors for use in Synechococcus: application to the identification of CO2-regulated promoters.

Author information

1
Department of Biological Sciences, University of Warwick, Coventry, U.K.

Abstract

It was shown that the Escherichia coli lacZ gene could be expressed in the cyanobacterium Synechococcus R2 PCC7942 both as a plasmid-borne form and also integrated into the chromosome. A promoterless form of the lacZ gene was constructed and used as a reporter gene to make transcriptional fusions with cyanobacterial promoters using a shuttle vector system and also via a process of integration by homologous recombination. Synechococcus R2 promoter-lacZ gene fusions were then used to identify CO2-regulated promoters, by quantitatively assessing beta-galactosidase activity under high and low CO2 conditions using a fluorescence assay. Several promoters induced under low CO2 conditions were detected.

PMID:
2116369
DOI:
10.1016/0378-1119(90)90437-v
[Indexed for MEDLINE]

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