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ISME J. 2011 Jun;5(6):1057-60. doi: 10.1038/ismej.2010.192. Epub 2010 Dec 16.

Phosphorus supply drives rapid turnover of membrane phospholipids in the diatom Thalassiosira pseudonana.

Author information

1
Ocean Biogeochemistry and Ecosystems, National Oceanography Centre, Southampton, UK. patrick.martin@noc.soton.ac.uk

Abstract

In low-phosphorus (P) marine systems, phytoplankton replace membrane phospholipids with non-phosphorus lipids, but it is not known how rapidly this substitution occurs. Here, when cells of the model diatom Thalassiosira pseudonana were transferred from P-replete medium to P-free medium, the phospholipid content of the cells rapidly declined within 48 h from 45±0.9 to 21±4.5% of the total membrane lipids; the difference was made up by non-phosphorus lipids. Conversely, when P-limited T. pseudonana were resupplied with P, cells reduced the percentage of their total membrane lipids contributed by a non-phosphorus lipid from 43±1.5 to 7.3±0.9% within 24 h, whereas the contribution by phospholipids rose from 2.2±0.1 to 44±3%. This dynamic phospholipid reservoir contained sufficient P to synthesize multiple haploid genomes, suggesting that phospholipid turnover could be an important P source for cells. Field observations of phytoplankton lipid content may thus reflect short-term changes in P supply and cellular physiology, rather than simply long-term adjustment to the environment.

PMID:
21160536
PMCID:
PMC3131859
DOI:
10.1038/ismej.2010.192
[Indexed for MEDLINE]
Free PMC Article

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