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Mol Immunol. 2011 Jan;48(4):415-22. doi: 10.1016/j.molimm.2010.09.003. Epub 2010 Dec 14.

MDA5 is SUMOylated by PIAS2β in the upregulation of type I interferon signaling.

Author information

1
Key Laboratory of Infection and Immunity of Chinese Academy of Sciences, the Institute of Biophysics, 15 Da Tun Road, Chaoyang District, Beijing 100101, China.

Abstract

Retinoic acid-inducible protein I (RIG-I) and melanoma differentiation-associated gene 5 (MDA5) are cytosolic viral RNA sensors that induce type I interferon production (IFN). In this study, we found that MDA5 undergoes inducible SUMOylation by small ubiquitin-like modifier-1 (SUMO-1) in response to polyI:C stimulation. Enhanced SUMOylation of MDA5 by exogenously expressed SUMO-conjugating enzyme Ubc9 correlated with upregulation of IFN expression and repressed virus replication. Conversely, overexpression of a SUMOylation-deficient mutant of Ubc9 or knockdown of endogenous Ubc9 reduced IFN production. Furthermore, we showed that PIAS2β, a SUMOylation E3 ligase, could specifically interact with and enhance the SUMOylation of MDA5. Consequently, PIAS2β knockdown reduced the SUMOylation of MDA5 and the IFN production. Collectively, these findings suggest that SUMO-1 modification of MDA5 possibly via PIAS2β may play a role in the MDA5-mediated IFN response to viral infections.

PMID:
21156324
PMCID:
PMC4091678
DOI:
10.1016/j.molimm.2010.09.003
[Indexed for MEDLINE]
Free PMC Article

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