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Langmuir. 2011 Jan 18;27(2):499-503. doi: 10.1021/la103632q. Epub 2010 Dec 14.

Free-standing biomimetic polymer membrane imaged with atomic force microscopy.

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1
Aquaporin A/S, Ole Maaløes Vej 3, DK-2200 Copenhagen N, Denmark.

Abstract

Fluid polymeric biomimetic membranes are probed with atomic force microscopy (AFM) using probes with both normal tetrahedrally shaped tips and nanoneedle-shaped Ag(2)Ga rods. When using nanoneedle probes, the collected force volume data show three distinct membrane regions which match the expected membrane structure when spanning an aperture in a hydrophobic scaffold. The method used provides a general method for mapping attractive fluid surfaces. In particular, the nanoneedle probing allows for characterization of free-standing biomimetic membranes with thickness on the nanometer scale suspended over 300-μm-wide apertures, where the membranes are stable toward hundreds of nanoindentations without breakage.

PMID:
21155606
DOI:
10.1021/la103632q
[Indexed for MEDLINE]
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