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Lab Chip. 2011 Mar 7;11(5):845-50. doi: 10.1039/c0lc00403k. Epub 2010 Dec 8.

Stand-alone self-powered integrated microfluidic blood analysis system (SIMBAS).

Author information

1
Biomedical Diagnostics Institute, National Centre for Sensor Research, Dublin City University, Glasnevin, Dublin 9, Ireland.

Erratum in

  • Lab Chip. 2011 Dec 21;11(24):4279. Park, Younggeun [added].

Abstract

We present a self-powered integrated microfluidic blood analysis system (SIMBAS) that does not require any external connections, tethers, or tubing to deliver and analyze a raw whole-blood sample. SIMBAS only requires the user to place a 5 μL droplet of whole-blood at the inlet port of the device, whereupon the stand-alone SIMBAS performs on-chip removal of red and white cells, without external valving or pumping mechanisms, followed by analyte detection in platelet-containing plasma. Five complete biotin-streptavidin sample-to-answer assays are performed in 10 min; the limit of detection is 1.5 pM. Red and white blood cells are removed by trapping them in an integral trench structure. Simulations and experimental data show 99.9% to 100% blood cell retention in the passive structure. Powered by pre-evacuation of its PDMS substrate, SIMBAS' guiding design principle is the integration of the minimal number of components without sacrificing effectiveness in performing rapid complete bioassays, a critical step towards point-of-care molecular diagnostics.

PMID:
21152509
DOI:
10.1039/c0lc00403k
[Indexed for MEDLINE]
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