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Mol Cell Biol. 2011 Feb;31(4):803-17. doi: 10.1128/MCB.01227-10. Epub 2010 Dec 13.

Reduction in ribosomal protein synthesis is sufficient to explain major effects on ribosome production after short-term TOR inactivation in Saccharomyces cerevisiae.

Author information

1
Universitaet Regensburg, Institut für Biochemie, Genetik und Mikrobiologie, Lehrstuhl Biochemie III, Regensburg, Germany.

Abstract

Ribosome synthesis depends on nutrient availability, sensed by the target of rapamycin (TOR) signaling pathway in eukaryotes. TOR inactivation affects ribosome biogenesis at the level of rRNA gene transcription, expression of ribosomal proteins (r-proteins) and biogenesis factors, preribosome processing, and transport. Here, we demonstrate that upon TOR inactivation, levels of newly synthesized ribosomal subunits drop drastically before the integrity of the RNA polymerase I apparatus is severely impaired but in good correlation with a sharp decrease in r-protein production. Inhibition of translation by cycloheximide mimics the rRNA maturation defect observed immediately after TOR inactivation. Both cycloheximide addition and the depletion of individual r-proteins also reproduce TOR-dependent nucleolar entrapment of specific ribosomal precursor complexes. We suggest that shortage of newly synthesized r-proteins after short-term TOR inactivation is sufficient to explain most of the observed effects on ribosome production.

PMID:
21149576
PMCID:
PMC3028636
DOI:
10.1128/MCB.01227-10
[Indexed for MEDLINE]
Free PMC Article

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